SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Coutinho,E.L.; Gomes,A.R.S.; França,C.N.; Salvini,T.F..

An alternative device for the immobilization of the hind limb of the rat was developed to study the effects of chronic disuse on the soleus and tibialis anterior muscles, maintained for 3 weeks in the shortening and the stretching positions, respectively. The proposed device is made of steel mesh and cotton materials, and has some advantages when compared to cast or plaster cast: it is cheaper, lighter (12 g or 4% of the body weight of the rat) and the same unit can be easily adjusted and used several times in the same animal or in animals of similar size. Immobilization is also useful to restrain the movements of the hip, knee, and ankle joints. Male rats (291 ± 35 g and aged 14 ± 2 weeks) were used to develop and test the model. The soleus muscle of 18...

ACL MYOTOXIN FROM Agkistrodon contortrix laticinctus ON FAST AND SLOW SKELETAL MUSCLE FIBERS OF MICE:ANALYSIS OF INJURY AND MUSCLE RECOVERY

Palavras-chave: Muscle immobilization; Muscle shortening; Muscle stretching; Soleus muscle; Tibialis anterior; Skeletal muscle.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100010

J. Venom. Anim. Toxins

Morini,C.C.; Pereira,E.C.L.; Araújo,H.S. Selistre de; Ownby,C.L.; Salvini,T.F..

Changes in types of muscle fibers induced by transcutaneous electrical stimulation of the diaphragm of rats

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200025

Costa,D.; Cancelliero,K.M.; Campos,G.E.R.; Salvini,T.F.; da Silva,C.A..

The objective of the present study was to assess the effect of transcutaneous electrical diaphragmatic stimulation (TEDS) on different types of diaphragm muscle fibers. Male Wistar rats (8-12 weeks old) were divided into 2 experimental groups (N = 8 in each group): 1) control, 2) animals submitted to TEDS [frequency = 50 Hz; T ON/T OFF (contraction/relaxation time) = 2/2 s; pulse duration = 0.4 ms, intensity = 5 mA with a 1 mA increase every 3 min for 20 min] for 7 days. After completing this treatment period, the I, IIA, IIB, and IID diaphragm muscle fibers were identified using the mATPase technique. Statistical analysis consisted of the normality, homoscedasticity and t-tests (P < 0.05). There was a 19.6% (P < 0.05) reduction in the number of type...

Cyclosporin-A does not affect skeletal muscle mass during disuse and recovery

Palavras-chave: Diaphragm; Transcutaneous electrical stimulation; Muscle fiber.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900011

Aoki,M.S.; Miyabara,E.H.; Soares,A.G.; Salvini,T.F.; Moriscot,A.S..

Cyclosporin-A (CsA) is an immunosuppressive drug that acts as an inhibitor of calcineurin, a calcium phosphatase that has been suggested to play a role in skeletal muscle hypertrophy. The aim of the present study was to determine the effect of CsA administration (25 mg kg-1 day-1) on skeletal muscle mass and phenotype during disuse and recovery. Male Wistar rats received vehicle (N = 8) or CsA (N = 8) during hind limb immobilization (N = 8) and recovery (N = 8). Muscle weight (dry/wet) and cross-sectional area were evaluated to verify the effect of CsA treatment on muscle mass. Muscle phenotype was assessed by histochemistry of myosin ATPase. CsA administration during immobilization and recovery did not change muscle/body weight ratio in the soleus (SOL)...

Decreased muscle strength is associated with proinflammatory cytokines but not testosterone levels in men with diabetes

Palavras-chave: Calcineurin; Immobilization; Recovery; Skeletal muscle; Hypertrophy; Cyclosporin-A.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200011

Ferreira,J.P.; Leal,A.M.O.; Vasilceac,F.A.; Sartor,C.D.; Sacco,I.C.N.; Soares,A.S.; Salvini,T.F..

The aim of this study was to compare muscle strength in male subjects with type 2 diabetes mellitus (DM2) with and without low plasma testosterone levels and assess the relationship between muscle strength, testosterone levels, and proinflammatory cytokines. Males (75) aged between 18 and 65 years were divided into 3 groups: control group that did not have diabetes and had a normal testosterone plasma level (>250 ng/dL), DnormalTT group that had DM2 with normal testosterone levels, and the DlowTT group that had DM2 and low plasma testosterone levels (<250 ng/dL). The age (means±SD) of the groups was 48.4±10, 52.6±7, and 54.6±7 years, respectively. Isokinetic concentric and isometric torque of knee flexors and extensors were analyzed by an isokinetic...

Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology

Palavras-chave: Diabetes; Testosterone; Muscle strength; Cytokines.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900610

Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F..

We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P...

Effect of passive stretching on the immobilized soleus muscle fiber morphology

Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005

Coutinho,E.L.; Gomes,A.R.S.; França,C.N.; Oishi,J.; Salvini,T.F..

The aim of the present study was to determine the effect of stretching applied every 3 days to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Eighteen 16-week-old Wistar rats were used and divided into three groups of 6 animals each: a) the left soleus muscle was immobilized in the shortened position for 3 weeks; b) during immobilization, the soleus was stretched for 40 min every 3 days; c) the non-immobilized soleus was only stretched. Left and right soleus muscles were examined. One portion of the soleus was frozen for histology and muscle fiber area evaluation, while the other portion was used to identify the number and length of serial sarcomeres. Immobilized muscles (group A) showed a significant decrease in weight...

Effects of exercise training on atrophy gene expression in skeletal muscle of mice with chronic allergic lung inflammation

Palavras-chave: Skeletal muscle; Passive stretch; Muscle immobilization; Sarcomere number; Muscle atrophy; Muscle fiber area.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200011

Durigan,J.L.Q.; Peviani,S.M.; Russo,T.L.; Silva,A.C.D.; Vieira,R.P.; Martins,M.A.; Carvalho,C.R.F.; Salvini,T.F..

We evaluated the effects of chronic allergic airway inflammation and of treadmill training (12 weeks) of low and moderate intensity on muscle fiber cross-sectional area and mRNA levels of atrogin-1 and MuRF1 in the mouse tibialis anterior muscle. Six 4-month-old male BALB/c mice (28.5 ± 0.8 g) per group were examined: 1) control, non-sensitized and non-trained (C); 2) ovalbumin sensitized (OA, 20 µg per mouse); 3) non-sensitized and trained at 50% maximum speed _ low intensity (PT50%); 4) non-sensitized and trained at 75% maximum speed _ moderate intensity (PT75%); 5) OA-sensitized and trained at 50% (OA+PT50%), 6) OA-sensitized and trained at 75% (OA+PT75%). There was no difference in muscle fiber cross-sectional area among groups and no difference in...

Functional changes of human quadriceps muscle injured by eccentric exercise

Palavras-chave: Exercise training; Muscle atrophy; Asthma; Gene expression; Mice.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400005

Serrão,F.V.; Foerster,B.; Spada,S.; Morales,M.M.B.; Monteiro-Pedro,V.; Tannús,A.; Salvini,T.F..

The present study evaluated functional changes of quadriceps muscle after injury induced by eccentric exercise. Maximal isometric torque of quadriceps and the surface electromyography (root mean square, RMS, and median frequency, MDF) of the vastus medialis oblique (VMO) and vastus lateralis (VL) muscles were examined before, immediately after and during the first 7 days after injury. Serum creatine kinase (CK) levels and magnetic resonance imaging (MRI) were used to identify muscle injury. The subject was used as her own control and percent refers to pre-injury data. Experiments were carried out with a sedentary 23-year-old female. Injury was induced by 4 bouts of 15 maximal isokinetic eccentric contractions (angular velocity of 5º/s; range of motion from...

Regenerated rat skeletal muscle after periodic contusions

Palavras-chave: Isometric torque; Surface electromyography; Quadriceps muscle; Muscle injury; Eccentric exercise; Magnetic resonance imaging.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600014

Minamoto,V.B.; Bunho,S.R.; Salvini,T.F..

In the present study we evaluated the morphological aspect and changes in the area and incidence of muscle fiber types of long-term regenerated rat tibialis anterior (TA) muscle previously submitted to periodic contusions. Animals received eight consecutive traumas: one trauma per week, for eight weeks, and were evaluated one (N = 8) and four (N = 9) months after the last contusion. Serial cross-sections were evaluated by toluidine blue staining, acid phosphatase and myosin ATPase reactions. The weight of injured muscles was decreased compared to the contralateral intact one (one month: 0.77 ± 0.15 vs 0.91 ± 0.09 g, P = 0.03; four months: 0.79 ± 0.14 vs 1.02 ± 0.07 g, P = 0.0007, respectively) and showed abundant presence of split fibers and fibers with...